WebNOTE:. For now, –circ and –tool options support results from CIRI2 / CIRCexplorer2 / DCC / KNIFE / MapSplice / UROBORUS / circRNA_finder / find_circ. For tools like DCC and circRNA_finder, please manually remove duplicated circRNAs with same junction postion but have opposite strands.. Gene expression values are needed for normalization, do not … WebApr 12, 2024 · The CIRI-long protocol combines rolling circular reverse transcription and nanopore sequencing to capture full-length circRNA sequences. After poly (A) tailing, RNase R treatment, and size ...
circtools · PyPI
WebCircular RNAs (circRNAs) are a recently discovered class of RNAs derived from protein-coding genes that have important biological and pathological roles. They are formed through backsplicing during co-transcriptional alternative splicing; however, the unified mechanism that accounts for backsplicing decisions remains unclear. WebCircular RNAs (CircRNAs) are a subclass of lncRNAs, which are transcribed and backspliced using the same cellular machinery as that by linear RNAs. In the past few years, circRNAs have been studied with … ray berryhill
circRNA-sponging: a pipeline for extensive analysis of circRNA ...
WebThe pipeline is built using Nextflow and processes data using the following steps: Raw read QC ( FastQC) Adapter trimming ( Trim Galore!) MultiQC report MultiQC circRNA quantification CIRIquant STAR 2-Pass mode CIRCexplorer2 circRNA finder DCC find circ MapSplice Segemehl circRNA annotation Export mature spliced length as FASTA file WebDec 31, 2024 · Circular RNA (circRNA) is a non-coding molecule produced through alternative splicing of one or more exons of a gene in the presence of an RNA-induced … WebDec 1, 2024 · Summary of computational pipelines for circRNA annotation and quantification. Names, aligners, links and references of these pipelines are provided. 2. … ray berry broward health