Cisplatin a549 xenograft

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August undefined, 2024

Weboma A549 cells and cisplatin (DDP) resistant A549 cells (A549DDP) were obtained from the American Type Culture ... xenograft tumor was visualized with the indicated antibodies. Ma et al. World Journal of Surgical Oncology (2015) 13:80 Page 4 of 6. A549/sCLU solid tumors (Figure 4). These findings sug-

TP53TG1 enhances cisplatin sensitivity of non-small cell lung …

WebDec 1, 2024 · Cisplatin-based chemotherapy is a common first-line regimen for treating non-small cell lung cancer (NSCLC). However, drug resistance is still a major problem. … WebJul 15, 2024 · The cisplatin-resistant A549 cell line (A549/DDP), the parent cell line A549, and 293 cells (JRDUN Biotech. Shanghai, China) were maintained in a humidified atmosphere with 5% CO 2 at 37 °C. phone roi from ni

Calu-6 Xenograft Model - Altogen Labs

WebMay 28, 2024 · In xenograft models, osimertinib significantly inhibited tumor growth, however, as expected, in 50% of mice drug-resistance developed. A combination of osimertinib with pemetrexed or cisplatin prevented or at … WebFeb 1, 2008 · Subcutaneous A549 xenograft model. A549 human lung adenocarcinoma cells (5 × 10 6) resuspended in 100 μL PBS were injected into the dorsal flank of 6-week-old to 8-week-old male BALB/c nude mice. When tumor size reached ∼50 mm 3, mice were randomized into three groups ( n = 6 per group). WebDec 1, 2024 · Xenograft Nephrotoxicity Antitumor effect 1. Introduction Cisplatin (CDDP) is a highly potent anticancer agent widely used for treating several types of cancers, … how do you set the time on a smart bracelet

Enhanced antitumor effect of cisplatin in human NSCLC cells by …

Cisplatin resistant lung cancer cells promoted M2 …

Webas subcutaneous xenograft model in mice. determination, provision of tumor tissue for target validation. Tumor volume, mean values +/- SEM. n=11. Figure 1: Growth of A549 cells Quality Assurance • Routine authentication of tumor cell lines by STR profiling • Mycoplasma testing of implanted tumor cells by PCR just prior to implantation Web2 days ago · Complex 11 showed a significant in vivo anticancer effect, revealed by its inhibiting effects on tumor growth in T24 xenograft mice without any renal pathological changes or influence on renal function. ... Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL … phone root toolWebThe A549 cell line was derived from a human lung adenocarcinoma and has been used in numerous studies to investigate various aspects of lung cancer biology, including cell … how do you set the time on a rolex datejust

"WebCisplatin: CDDP: A549, H358, H460 and Calu-1 cells: ... H460, H661, A549 cells and xenograft mouse models: Upregulating TF and TFR, downregulating SLC7A11 in a p53-dependent manner and increasing intracellular Fe 2+, … " - Cisplatin a549 xenograft

Cisplatin a549 xenograft

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Weboma A549 cells and cisplatin (DDP) resistant A549 cells (A549DDP) were obtained from the American Type Culture ... xenograft tumor was visualized with the indicated … WebJan 20, 2024 · Deferoxamine Counteracts Cisplatin Resistance in A549 Lung Adenocarcinoma Cells by Increasing Vulnerability to Glutamine Deprivation-Induced Cell Death . Authors Wen-Jun Liu 1 2 , Peng-Yu Pan 2 , Ye Sun 3 , Jian-Bo Wang 4 , Huan Zhou 2 , Xin Xie 1 , Zhi-Yuan Duan 1 , Han-Yu Dong 2 , Wen-Na Chen 4 , Li-de Zhang 2 , …

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WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … WebDec 1, 2024 · A549 cells, the human NSCLC cells with inherent cisplatin resistance, were used to investigate synergistic effect of sclareol with cisplatin in cell proliferation and migration as well as its regulatory mechanisms in expression of excision repair cross-complementation group 1 (ERCC1), a cisplatin resistance-associated molecule.

WebSep 18, 2024 · NCBI Bookshelf WebJan 12, 2024 · Here, we show that this combination enhances the anti-cancer effect of cisplatin in both in vitro cell lines and in vivo xenograft mouse models of cisplatin-sensitive and -resistant lung, ovarian ...

WebJul 15, 2024 · A549/DDP cells with TIMR59 knockdown was more sensitive to cisplatin treatment in a xenograft model. Moreover, A549/DDP cells exhibited increased glucose … WebOct 7, 2013 · A Balb/c nude mouse xenograft model using A549 cells was previously established. The mice were randomly divided into normal, mock, Ke, cisplatin (DDP), and co-formulated (Ke + DDP) groups. After 15 days of drug administration, the animals were sacrificed, body weight and tumor volume were recorded, and the tumor-inhibiting rate …

WebDec 1, 2024 · Cisplatin-based chemotherapy is a common first-line regimen for treating non–small cell lung cancer (NSCLC). However, drug resistance is still a major problem. The purposes of this study were to evaluate whether sclareol can reverse cisplatin resistance and to investigate its possible mechanisms.

WebA standard immunohistochemistry (IHC) protocol was used to analyze tumor samples harvested from the A549R xenograft experiment. Briefly, the sections (5μm-thick) were first dewaxed and re-hydrated, and the endogenous peroxidase activity was blocked using 3% hydrogen peroxide. phone rom meaningWebTargeting of the nano‐zinc carrier to A549/DDP or A549 cells in vivo was detected after subcutaneous injection in a mouse xenograft tumor model. A549/DDP or A549 cells (1 × 10 6 cells) in 50 μL of PBS were injected into the armpits of 4‐week‐old nude mice (BALB/c, nu/nu; SIPPR‐BK Laboratory Animal Co. Ltd, Shanghai, China) using a 25 ... phone rooms in officeWebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug Association in 1978.7Platinum chemotherapy drugs, represented by … how do you set timeWebMar 17, 2024 · Human NSCLC cell lines (A549, H1299, H1650, SK-MES-1, and Calu-3) and a normal human bronchial epithelial cell line (HBE) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI-1640 medium at 37 °C in a humidified atmosphere of 5 % CO 2. how do you set the timerWebApr 8, 2014 · The MTT assay demonstrated that cisplatin exhibits a dose‑dependent cytotoxicity in A549 cells and synergizes with radiation to promote the cell‑killing effect of radiation. In the xenograft mouse model of Lewis cells, cisplatin plus ionizing radiation (IR) (five doses of 2 Gy) yielded the most significant tumor suppression. phone room thermometerWebas subcutaneous xenograft model in mice. determination, provision of tumor tissue for target validation. Tumor volume, mean values +/- SEM. n=11. Figure 1: Growth of A549 … how do you set the time on an amazfit watchWebSep 14, 2024 · A549 cells were seeded in 96-well plates and cultured for 24 hours, then treated with varying concentrations of rhMG53 (0.05-100 μg/ml) or rhMG53 plus cisplatin (5 μM) for either 48 hours or 72 hours. Cells were detached by 0.25% Trypsin-EDTA solution, and their viability were quantified by flow cytometry (CytoFLEX S system, Beckman … phone roof