Ctll 2 assay

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August undefined, 2024

WebThe ED 50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10 7 IU/mg. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB, pH3.5, with 5 % ... WebFeb 13, 2013 · A toxicogenomics approach was applied to assess the usefulness of the mouse cytotoxic T cell line CTLL-2 for in vitro immunotoxicity testing. CTLL-2 cells were exposed for 6 h to two model immunotoxic compounds: (1) the mycotoxin deoxynivalenol (DON, 1 and 2 μM), a ribotoxic stress inducer, and (2) the organotin compound tributyltin …

Antigen presentation by mouse CD4 - American Society of …

WebCTLL, CTLL2, CTLL(2), CT, CTL2, CTL-2, TLL 2 Mouse C57b1/6 T cell CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Mouse Blood Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells. Nature 268:154-6 PMID: 145543 … http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf grahams equation

Measurement of human and murine interleukin 2 and …

WebPreparation of CTLL-2 Cell Assay Medium Reagent Amount . RPMI 1640 medium (3.1) 450 mL . Heat-inactivated FBS (3.2) 50 mL . Label as CTLL-2 Cell Assay Medium with the … WebNational Center for Biotechnology Information WebSupport protocols are provided for the quantitation of CTLL-2, CT4.S, or CT.h4S proliferation using a standard [3H]thymidine incorporation method or by using the 3-(4,5 … graham service center

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CTLL assay: Comparison of two methods for IL-2 …

WebCellGenix® GMP rh IL-2 has an activity of 8 – 32 × 106 IU/mg. The activity of GMP rh IL-2 was measured in a cell proliferation assay using the IL-2-dependent cell line CTLL-2. It was calibrated against Proleukin®. You can find the batch specific activity on … WebThe kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required. ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. graham servicesWebCTLL-2 cells (50,000 cells/well) were plated the day of the assay in a 384-well assay plate. mIL-2 was then added to the plate over the indicated concentration range. Plates … grahams estate agents whitchurch

"WebApr 1, 2003 · The ability of 3A9 cells to present the acquired MHC II:peptide complexes to another T cell with the same antigen specificity was determined by their ability to induce IL-2 production by the fresh responder cells using the CTLL-2 bioassay. Figure 4A shows the positive control results of culturing 3A9 cells with the DAP.3 H2-A k transfectant as ... " - Ctll 2 assay

Ctll 2 assay

WebJun 2, 2012 · Another well-known bioassay utilizes a CTLL-2 cell line as a target for measuring proliferative activity of IL-2 (Gillis et al., 1978) ... The selection of the … WebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. 培地 RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human …

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WebNov 29, 2013 · 2.2. Assay methods and study design. ... CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell … WebThe IL-15 Bioassay is a bioluminescent cell-based assay designed to measure IL-15 stimulation or inhibition. The IL-15 Bioassay Cells have been engineered to express luc2 in response to IL-15 signaling. When IL-15 binds to IL-15 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence.

WebNov 22, 2007 · It relies on the IL-2- or IL-4-dependence of a cytotoxic cell line (CTLL-2) for in vitro proliferation, and the measurement. of the growth of the these cells is usually … WebCellTiter-Glo 2.0 Assay will maintain >90% activity upon storage at 4°C for 4 weeks or >85% activity upon storage at room temperature for 7 days. The CellTiter-Glo 2.0 Assay …

WebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. WebMay 18, 2024 · CTLL-2 cells (AATC TIB-214), a murine IL-2-dependent T cell line, were obtained from the vendor and maintained in complete RPMI supplemented with 1000 IU/mL recombinant mouse IL-2. ... Furthermore, the in vitro suppression assay graph in Figure 6—figure supplement 1 is insufficient without a "No Treg" control to evaluate the degree …

WebPromega ctll 2 proliferation assay Ctll 2 Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebApr 1, 2024 · CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 pM. 3.4. Prolonged half-life of IL-15·sIL-15Rα/Fc. The half-life of circulating IL-15 in blood is critical for its in vivo bioactivities. china house menu huntsville ontarioWeb6.4 Label the complete medium as CTLL-2 Cell Culture Medium with the lot number, date prepared, initials, and expiry date based on the component with the shortest expiration … china house menu holly hillWebFeb 1, 1997 · Almost identical results were obtained when the IL-2 levels were detected by the conventional CTLL assay, in which DNA synthesis was monitored by 3H-thymidine labeling, and by the isotope-free MTT ... grahams facilities managementWebFeb 24, 2024 · CTLL-2 proliferation assay. In order to starve the CTLL-2 cells from IL-2, the cells were washed twice with prewarmed HBSS (Gibco, #14175095) and then grown in … graham services limitedWebOct 31, 2000 · In this study, alamarBlue was used to measure the proliferation of the IL-2-dependent cytotoxic T cell line, CTLL-2. The colorimetric change of alamarBlue at 570 nm compared to the reference wavelength, 600 nm, was proportional to the number of viable cells. The sensitivity of the IL-2 assay using alamarBlue was comparable to that of the … china house menu kingsport tnWebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human … grahams evesham new showroomWebApr 7, 2024 · The CTLL-2 cell proliferation assay is the standard assay to calculate the biological activity of IL-2, measured in IU/mg 25. We compared S2A and the clinical … grahams express transport