Stat3 reporter assay
WebLuciferase Reporter Assay. The regulatory effects of miR-525-5p on Stat-1 were investigated by luciferase reporter assays. Wild-type and mutant 3′-UTR sequences of Stat-1 were cloned into the pGL-3M recombinant plasmid. U251 and T98 cells overexpressing miR-525-5p or control were transfected with the luciferase reporters using Lipofectamine 3000. WebMar 15, 2024 · O-GlcNAcglycosylation, JAK2-STAT3 pathway Introduction Esophageal squamous cell carcinoma (ESCC), the fourth leading cause of cancer death in China, is a highly aggressive malignant tumor [1]. It has high morbidity and mortality with less than 50% of 5-year survival rate and increasing incidence in recent years.
Stat3 reporter assay
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WebBioinformatics prediction, luciferase reporter assay, and pull-down assay were used to determine if miR-149 was a target of GACAT3. In addition, we also conducted colony formation assays and invasion assays to verify that GACAT3 promotes tumor progression through miR-149. ... Keywords: GACAT3, miR-149, colorectal cancer, growth, metastasis, … WebSignosis has established Stat3 luciferase reporter HEK293 stable cell line, which can be used as a reporter system for monitoring the activation of Stat3 triggered by stimuli …
WebAug 2, 2024 · STAT3 has been shown to be a cancer driver and a modulator of the tumor microenvironment. As such, it has become an attractive target for therapeutic drugs and … WebAntibodies that detect STAT3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and …
WebSTAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), forms homo- or …
WebJan 8, 2024 · The configuration and use of the STAT3 SH2 domain specific fluorescence polarization and STAT3-response luciferase reporter assays allowed the screening of N4 …
WebReporter Assays State-of-the-art bioluminescent assays. Detect firefly, Renilla, NanoLuc® and click-beetle luciferase activities. Transfection Reagents Proven, well-cited transfection solutions for a range of cell types, including primary cells and iPSCs. Top Luciferase Assay Products for Your Lab Nano-Glo® Dual-Luciferase Reporter Assay System sdn nicu fellowshipWebThese cell lines contain STAT response element that controls reporter gene expression, and can be used to study stimulation or inhibition of STAT transcription factors. Functional … peace lutheran preschool el paso txWebDec 8, 2024 · In the cell-based functional assay, SI-109 effectively inhibits the transcriptional activity of STAT3 in a STAT3-luciferase reporter assay (IC 50 =3 μM). SD-36 inhibits the MOLM-16 cell line with an IC 50 of 35 nM. Moreover, SD-36 … peace lutheran preschool waunakee wiWebDescription The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase … peace lutheran rogers arWebCignal Lenti Reporter Assays are ready-to-transduce lentiviral particles that enable the measurement of a signaling pathway in any mammalian cell. Pathway-specific reporters … peace lutheran school calendar saginaw miWebA cell based NF-κB reporter assay indicated that TROY mediated NF-κB activation which was not inhibited by ruxolitinib treatment. The immunoblots (lower panel) of these samples showed the activation of STAT3 by TROY and TROY-TRAFm variant as well as the equivalent expression of TROY and TROY-TRAFm variant. ***, p < 0.001. peace lutheran school antigoWebGenetic reporter assays are based on reporter gene expression in response to activation of specific signaling cascade, followed by monitoring the presence of the reporter protein by directly measuring its enzymatic activity. sdn medical school interview questions