WebThe protocol describes the MSD GOLD -TAG conjugation SULFOprocedure for proteins with a MW > 40000 Da. Smaller , proteins/polypeptides may also be conjugatedusing MSD GOLD SULFO-TAG NHS-Ester as long as they have an accessible lysine or the N-terminal amino group; however, alternative separation methods may be needed to remove unconjugated … WebJan 10, 2012 · N-(2-ferrocene-ethyl)maleimide (FEM, 20, Fig. 7) is an electroactive precolumn tag which can be used for thiol quantification . Thiol 6 adds across the double bond of 20, forming the thiol conjugate 21. The iron core of the ferrocene moiety readily undergoes oxidation, from Fe 0 to Fe +, in the ionisation source of an ESI-MS .

Oriented Immobilization of Farnesylated Proteins by the Thiol-Ene …

WebThe His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. These residues readily coordinate … WebDevelopment of a novel biotin thiol tag for citrullination analysis. Protein citrullination and homocitrullination both feature a ureido group on the side chains that can be used for chemical derivatization as previously reported 6, 22, 34, 35.Here, we design a biotin thiol tag that can be easily synthesized with low cost (Supplementary Fig. 1) and can specifically … hammock with net

Iodoacetyl Tandem Mass Tag™ (iodoTMT™) Reagents

WebMay 13, 2015 · Herein, we report the design and development of a biotin thiol tag that enables derivatization, enrichment, and confident identification of these two PTMs simultaneously via mass spectrometry. We ... Web多肽标签(Tag) 酶底物肽; 抑制剂相关肽; 淀粉样肽(Amyloid Peptides) 激动剂多肽(agonist peptide) ADC Linker; 抗菌肽(Antimicrobial Peptides) 环二肽; 二肽(Dipeptide) 三肽; 糖肽(GlcNAc) 自组装相关肽; 更多 WebDownload scientific diagram ฀Design of biotin thiol tag for citrullination and homocitrullination analysis. a , PAD enzymes catalyze the hydrolytic conversion of peptidyl arginine residues ... hammock with stand 500 weight capacity